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| Registros recuperados: 69 | |
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| Oliveira,E.C.S.; Juliani,G.C.; Marques Jr.,A.P.; Henry,M.. |
| The efficacy of three extenders, tris-egg yolk-5% ethylene glycol (T1), lactose-egg yolk-5% ethylene glycol (T2) and lactose-egg yolk-5% dimethyl formamide (T3) on preserving the viability of post-thawing canine spermatozoa was evaluated. Three ejaculates per dog were obtained of five animals. The semen was packaged in 0.5ml straws and cooled to 4°C for 120min. The straws were frozen 4cm above the nitrogen level for 15min and thawed in water-bath at 37°C for 60sec and at 75°C for 7sec. Progressive motility and vigour were evaluated immediately after thawing (time 0) and at 30, 60, 90 and 120min. Structural and functional integrity of plasma membrane of the spermatozoa were evaluated, respectively, by fluorescent staining probes and hypoosmotic swelling... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Semen; Cryopreservation; Ethylene glycol; Dimethyl formamide. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600021 |
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| Horn, Karin; Ono, Kenichiro; Heydorn, Alfred Otto. |
| This paper describes a standard method for in vitro excystation of sporocysts of S.capracanis, S.hircicanis, S.ovicanis and S.arieticanis. A standard pretreatment was performed by 20 minutes preincubation of sporocysts in aqueous sodium hypochlorite (NaOCl) solution of 6% or 8% at room temperature. Then, sporocysts were washed 5 times in distilled water and were incubated for one hour at 39OC in excystation fluid consisting of RPMI 1640 medium, 10% PCS and 15% bovine bile. Additional sonication of pretreated sporocysts increased excystation rates as compared to not sonicated controls. Excystation rates for 1-9 months old sporocysts were 77% for S.capracanis, 77% for S.hircicanis, 72% for S.arieticanis and 92% for S.ovicanis. Sporozoite suspension as... |
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Palavras-chave: Cryopreservation; In vitro excystation; Sarcocystis spp.; Sporozoites. |
| Ano: 1991 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/154 |
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| Hariya, Madoka; Suzuki, Hiroshi. |
| Although vitrification and follicular survival subsequent to xenotransplantation of canine ovarian tissues have been reported, the degree of cryoinjury after the vitrification has not yet been fully examined. Since cryoinjury after vitrification of ovaries has been reported in bovine and humans, this study evaluated canine ovarian tissues after vitrification by immunohistochemistry and TUNEL assay. Ovaries were collected from immature bitches. The ovarian tissue cubes were pretreated with 1 M DMSO. Subsequently, DAP 213 solution (2 M DMSO, 1 M acetamide, 3 M propylene glycol) was added. The cryotubes containing the ovarian tissues were placed on ice for 5 min, then plunged directly into liquid nitrogen. After warming with 0.25 M sucrose, cryopreserved... |
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Palavras-chave: Cryopreservation; Dog; Follicle; Ovary. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4535 |
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| Suquet, Marc; Dreanno, Catherine; Petton, Bruno; Normant, Yvon; Omnes, Marie-helene; Billard, Roland. |
| The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use... |
| Tipo: Text |
Palavras-chave: Turbot; Psetta maxima; Sperm; Cryopreservation; Hatching. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00176/28721/27176.pdf |
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| Olmos-Zúãiga,J.R.; Jasso-Victoria,R.; Díaz-Martínez,N.E.; Gaxiola-Gaxiola,M.O.; Sotres-Vega,A.; Heras-Romero,Y.; Baltazares-Lipp,M.; Baltazares-Lipp,M.E.; Santillán-Doherty,P.; Hernández-Jiménez,C.. |
| Various methods are available for preservation of vascular grafts for pulmonary artery (PA) replacement. Lyophilization and cryopreservation reduce antigenicity and prevent thrombosis and calcification in vascular grafts, so both methods can be used to obtain vascular bioprostheses. We evaluated the hemodynamic, gasometric, imaging, and macroscopic and microscopic findings produced by PA reconstruction with lyophilized (LyoPA) grafts and cryopreserved (CryoPA) grafts in dogs. Eighteen healthy crossbred adult dogs of both sexes weighing between 18 and 20 kg were used and divided into three groups of six: group I, PA section and reanastomosis; group II, PA resection and reconstruction with LyoPA allograft; group III, PA resection and reconstruction with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pulmonary artery graft; Cryopreservation; Lyophilization; Blood vessel bioprosthesis; Prosthesis design; Vascular procedures. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200701 |
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| Suquet, Marc; Malo, Florent; Gourtay, Clemence; Ratiskol, Dominique; Riobe, Marion; Le Grand, Jacqueline; Queau, Isabelle; Mingant, Christian. |
| A basic cryopreservation protocol for great scallop sperm was recently published, with which thawed sperm survival remained limited to 25% of control values (Suquet et al. 2014). We designed here a set of five experiments to test ways of improving thawed sperm survival and storage by studying the effects of (1) cryoprotectant concentration; (2) sugar incorporation in the extender; (3) egg yolk addition to the extender; (4) sperm dilution rate; and (5) storage capacity of thawed sperm at 4 degrees C. A sixth experiment was then performed to compare rearing performances of larvae produced using thawed sperm from the optimal protocol defined in the present study with those produced using fresh sperm. During the first experiment, a significantly higher... |
| Tipo: Text |
Palavras-chave: Pecten maximus; Mollusc; Sperm; Cryopreservation; Larval rearing. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00359/47033/46946.pdf |
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| Sprícigo,J.F.W.; Leme,L.O.; Guimarães,A.L.; Oliveira Neto,J.C.; Silva,P.C.P.; Moreira,N.H.; Pivato,I.; Silva,B.D.M.; Ramos,A.F.; Dode,M.A.N.. |
| ABSTRACT Piau porcine blastocysts were submitted to MALDI-TOF to identify the main phospholipids (PL). After that, in vivo blastocysts (D6) were vitrified (n=52), non-vitrified were used as control (n=42). After warming, blastocysts were in vitro cultured to assess re-expansion and hatching at 24 and 48 hours. Finally, at 48 hours, hatched blastocysts were submitted to RT-qPCR searching for BCL2A1, BAK, BAX and CASP3 genes. For MALDI-TOF, the ion intensity was expressed in arbitrary units. Blastocyst development was compared by Qui-square (P< 0.05). Among the most representative PL was the phosphatidylcholine [PC (32:0) + H]+; [PC (34:1) + H]+ and [PC (36:4) + H]+. Beyond the PL, MALDI revealed some triglycerides (TG), including PPL (50:2) + Na+, PPO... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pig; Embryo; Cryopreservation; Lipids; Apoptosis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300837 |
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| Hernández,P.J.E; Fernández,R.F; Rodríguez,S.J.L; Soto,M.Y.G; Verona,J.E.H; García,R.A.D. |
| In order to evaluate the post-thaw effect on acrosomal viability and reaction of sperm collected from equine epididymis tail, 62 testes were collected and transported at 4°C. Epididymis was dissected to perform the retrograde flushing of the epididymis tail , using 10 mL of a diluent based on skim milk (INRA 82). The diluent obtained with the spermatic content was recovered in graduated collecting tubes and 1mL of each sample was taken to be evaluated. For evaluations, the samples having a minimum of 30% of progressive motility were selected. The evaluations were on: percentage of live sperm, normal morphology, sperm concentration, percentage of live sperm without acrosomal reaction (VsRA) and live with acrosomal reaction (VcRA), evaluating this indicator... |
| Tipo: Journal article |
Palavras-chave: Cryopreservation; Viability; Acrosomal reaction; FITC-PNA/IP; Sperm; Epididymis; Equine. |
| Ano: 2012 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2012000200004 |
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| Demoy-schneider, Marina; Schmitt, Nelly; Le Pennec, Gael; Suquet, Marc; Cosson, Jacky. |
| High quality of sperm is essential to a high fertilization rate, especially post- cryopreservation. Assessment of sperm integrity, motility and energy reserves before cryopreservation is necessary for selection of milt with optimal fertilizing potential. We describe the effect of cryopreservation on the quality of black-lip pearl oyster, Pinctada margaritifera var. cumingii sperm. Evaluated quality indices of fresh and frozen/thawed P. margaritifera spermatozoa, included morphology, ultrastructure and motility characteristics relative to the energy content (ATP) and its capacity to be sustained by mitochondrial respiration. Morphology and ultrastructure were quantitatively evaluated using images obtained by optical microscopy assisted by the Image J... |
| Tipo: Text |
Palavras-chave: Spermatozoa quality; Cryopreservation; Pinctada margaritifera; Motility; Metabolism; CASA. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00453/56421/58114.pdf |
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| 鈴木, 宏志. |
| Although guide dogs make a remarkable contribution to the quality of life of the blind people, many countries suffer from an acute shortage of guide dogs such that not all those who need them are able to be supplied. One of the important challenges from the point of view of animal and veterinary science as well as social welfare is to establish a stable and effective breeding and reproduction system for guide dogs for the blind. Although the development and extension of assisted reproductive techniques such as artificial insemination, embryo transfer and in vitro fertilization has been remarkable, in canines, it is not a successful as other mammalian spices such as mice, pigs, cows and humans. In this review, recent advances of cryopreservation of sperm,... |
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Palavras-chave: Guide dog; Artifical insemination; Embryo transfer; Recipient; Cryopreservation; イヌ; 人工授精; 移植; 受容雌; 凍結保存. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4511 |
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| Ninhaus-Silveira,Alexandre; Foresti,Fausto; Veríssimo-Silveira,Rosicleire; Senhorini,José Augusto. |
| Aiming to improve fish reproduction techniques, the characterization and cryopreservation of semen of Brycon cephalus were performed. The seminal characteristics observed were: an almost transparent, milky semen with a mean volume of 4mL, and sperm concentration of 9.617±1.630 x 10(6) spermatozoa/mm³. Spermatozoa (length = 31.288 ±4.47µm) were of the aquasperm type and displayed a small, round head (length = 1.727 ±0.18 µm; width = 1.752 ±0.17µm) without acrosomal vesicle, nucleus with highly condensed chromatin forming coarse clots and centriolar complex located in the nuclear fossa; a midpiece (length = 2.561±0.44µm), narrowed rearward, with a cytoplasmic canal; and a flagellum (length = 29.521 ±4.37µm). Fertilization tests with thawed semen demonstrated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Semen characteristics; Cryopreservation; Brycon cephalus; Ultrastructure; Fish. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500015 |
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| Suquet, Marc; Le Mercier, Alain; Rimond, Flore; Mingant, Christian; Haffray, Pierrick; Labbe, C.. |
| Parameters used to assess the survival of larvae after cryopreservation generally misestimate the damages that prevent larval development. The objectives of the present study were to 1) define the reliability of the survival rate, assessed at 2 and 7 days post fertilization, to estimate Pacific oyster larval quality after thawing, and 2) select complementary tools allowing an early and reliable estimation of their quality. Oyster larvae were reared for 25 h after fertilization at 19 degrees C and cryopreserved at early D-stage. Then, thawed larvae were incubated in 2-L beakers. At 2 days post fertilization, the survival rate of thawed Pacific oyster larvae was lower than that of fresh larvae for only one experiment (Experiment 3) among the four identical... |
| Tipo: Text |
Palavras-chave: ASMA; CASA; Crassostrea gigas; Cryopreservation; D-larvae. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00088/19934/17761.pdf |
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| Groison, Anne-laure; Suquet, Marc; Cosson, Jacky; Mayer, Ian; Severe, Armelle; Bouquet, Jean-marie; Geffen, Audrey J.; Utne-palm, Anne Christine; Kjesbu, Olav Sigurd. |
| Due to stock declines there is a growing interest in farming of European hake Merluccius merluccius. Thus, knowledge of its sperm biology is of importance not only for purposes of broodstock management, but also for the development of sperm preservation techniques. Hake sperm were collected from mature males caught during the summer-early autumn waters off western Norway and during the winter-early spring in the Bay of Biscay (France). Sperm quality characteristics were assessed after storage at 4 degrees C for 25 +/- 14 h. Average (+/- SD) values for Norwegian and French samples respectively were (i) sperm volume: 3.9 +/- 4.0 and 2.6 +/- 4.0 ml; (ii) spermatozoa concentration: 6.6 +/- 3.2 and 13.9 +/- 5.1 x 10(9) spermatozoa/ml; (iii) spermatocrit: 80.2... |
| Tipo: Text |
Palavras-chave: Cryopreservation; Merluccius merluccius; Osmolality; PH; Sperm motility; Spermatocrit. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00003/11393/8021.pdf |
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| Tiersch,Terrence R.. |
| Initial success in sperm cryopreservation occurred at about the same time for aquatic species and livestock. However, in the 50 plus years since then cryopreserved sperm of livestock has grown into a billion-dollar global industry, while cryopreserved sperm of aquatic species remains a research activity with little commercial application despite work in more than 90 species and more than 200 published reports. Most research work has focused on large-bodied culture and sport fishes, such as salmon, trout, carp, and catfish, and mollusks such as commercially important oyster and abalone species. However, only a few studies have addressed sperm cryopreservation in small fishes such as zebrafish, or in endangered species. Overall, this work has yielded... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquatic species; Cryopreservation; Germplasm; Repositories. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982008001300003 |
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| Registros recuperados: 69 | |
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